Tag Archives: brian

This artist’s impression depicts the formation of a galaxy cluster in the early Universe. The galaxies are vigorously forming new stars and interacting with each other. Such a scene closely resembles the Spiderweb Galaxy (formally known as MRC 1138-262) and its surroundings, which is one of the best-studied protoclusters.

Credit:

ESO/M. Kornmesser

Universe may face a darker future

Since the discovery of the accelerated expansion of the universe in 1997 by High-Z Supernova Team led by Prof. Brian Schmidt and Adam Rees, and by Supernova Cosmology Project Team led by Prof. Saul Perlmutter, the question of the nature of this expansion and the role of the mysterious dark energy has puzzled the minds of many theoretical and observational physicists/astrophysicists.

Another puzzling question in astronomy comes from the unusual behavior of the stars revolving around the galaxies with higher velocities than expected if we consider the apparent baryonic matter in the galaxy.This has led to many new questions related to something we called the dark matter, another unexplained phenomenon.

 


 

New research offers a novel insight into the nature of dark matter and dark energy and what the future of our Universe might be.

Researchers in Portsmouth and Rome have found hints that dark matter, the cosmic scaffolding on which our Universe is built, is being slowly erased, swallowed up by dark energy.

The findings appear in the journal Physical Review Letters, published by the American Physical Society. In the journal cosmologists at the Universities of Portsmouth and Rome, argue that the latest astronomical data favours a dark energy that grows as it interacts with dark matter, and this appears to be slowing the growth of structure in the cosmos.

Professor David Wands, Director of Portsmouth’sInstitute of Cosmology and Gravitation, is one of the research team.

He said: “This study is about the fundamental properties of space-time. On a cosmic scale, this is about our Universe and its fate.

“If the dark energy is growing and dark matter is evaporating we will end up with a big, empty, boring Universe with almost nothing in it.

 

“Dark matter provides a framework for structures to grow in the Universe. The galaxies we see are built on that scaffolding and what we are seeing here, in these findings, suggests that dark matter is evaporating, slowing that growth of structure.”

Cosmology underwent a paradigm shift in 1998 when researchers announced that the rate at which the Universe was expanding was accelerating. The idea of a constant dark energy throughout space-time (the “cosmological constant”) became the standard model of cosmology, but now the Portsmouth and Rome researchers believe they have found a better description, including energy transfer between dark energy and dark matter.

Research students Valentina Salvatelli and Najla Said from the University of Rome worked in Portsmouth with Dr Marco Bruni and Professor Wands, and with Professor Alessandro Melchiorri in Rome. They examined data from a number of astronomical surveys, including the Sloan Digital Sky Survey, and used the growth of structure revealed by these surveys to test different models of dark energy.

Professor Wands said: “Valentina and Najla spent several months here over the summer looking at the consequences of the latest observations. Much more data is available now than was available in 1998 and it appears that the standard model is no longer sufficient to describe all of the data. We think we’ve found a better model of dark energy.

“Since the late 1990s astronomers have been convinced that something is causing the expansion of our Universe to accelerate. The simplest explanation was that empty space – the vacuum – had an energy density that was a cosmological constant. However there is growing evidence that this simple model cannot explain the full range of astronomical data researchers now have access to; in particular the growth of cosmic structure, galaxies and clusters of galaxies, seems to be slower than expected.”

Professor Dragan Huterer,of the University of Michigan, has read the research and said scientists need to take notice of the findings.

He said: “The paper does look very interesting. Any time there is a new development in the dark energy sector we need to take notice since so little is understood about it. I would not say, however, that I am surprised at the results, that they come out different than in the simplest model with no interactions. We’ve known for some months now that there is some problem in all data fitting perfectly to the standard simplest model.”

Source: Materials taken from Uop News

Bioengineers create functional 3-D brain-like tissue

Tissue model could change the way scientists study the brain in vitro-NIH study

Bioengineers have created three-dimensional brain-like tissue that functions like and has structural features similar to tissue in the rat brain and that can be kept alive in the lab for more than two months.

As a first demonstration of its potential, researchers used the brain-like tissue to study chemical and electrical changes that occur immediately following traumatic brain injury and, in a separate experiment, changes that occur in response to a drug. The tissue could provide a superior model for studying normal brain function as well as injury and disease, and could assist in the development of new treatments for brain dysfunction.

Confocal microscope image of neurons (greenish yellow) attached to silk-based scaffold (blue). The neurons formed functional networks throughout the scaffold pores (dark areas). Image courtesy of Tufts University.
Confocal microscope image of neurons (greenish yellow) attached to silk-based scaffold (blue). The neurons formed functional networks throughout the scaffold pores (dark areas). Image courtesy of Tufts University.

The brain-like tissue was developed at the Tissue Engineering Resource Center at Tufts University, Boston, which is funded by the National Institute of Biomedical Imaging and Bioengineering (NIBIB) to establish innovative biomaterials and tissue engineering models.  David Kaplan, Ph.D., Stern Family Professor of Engineering at Tufts University is director of the center and led the research efforts to develop the tissue.

Diagram of scaffold donut showing grey-white matter compartmentalization. Rat neurons attached to the scaffold (donut ring) and also sent axons (labeled with green fluorescence) through the collagen gel-filled center.
Diagram of scaffold donut showing grey-white matter compartmentalization. Rat neurons attached to the scaffold (donut ring) and also sent axons (labeled with green fluorescence) through the collagen gel-filled center.

Currently, scientists grow neurons in petri dishes to study their behavior in a controllable environment. Yet neurons grown in two dimensions are unable to replicate the complex structural organization of brain tissue, which consists of segregated regions of grey and white matter. In the brain, grey matter is comprised primarily of neuron cell bodies, while white matter is made up of bundles of axons, which are the projections neurons send out to connect with one another. Because brain injuries and diseases often affect these areas differently, models are needed that exhibit grey and white matter compartmentalization.

Recently, tissue engineers have attempted to grow neurons in 3D gel environments, where they can freely establish connections in all directions. Yet these gel-based tissue models don’t live long and fail to yield robust, tissue-level function. This is because the extracellular environment is a complex matrix in which local signals establish different neighborhoods that encourage distinct cell growth and/or development and function.  Simply providing the space for neurons to grow in three dimensions is not sufficient.

Now, in the Aug. 11th early online edition of the journal Proceedings of the National Academy of Sciences, a group of bioengineers report that they have successfully created functional 3D brain-like tissue that exhibits grey-white matter compartmentalization and can survive in the lab for more than two months.

“This work is an exceptional feat,” said Rosemarie Hunziker, Ph.D.

Image of silk-based scaffold taken with a scanning electron microscope reveals its porous, sponge-like composition. Image courtesy of Tufts University.
Image of silk-based scaffold taken with a scanning electron microscope reveals its porous, sponge-like composition. Image courtesy of Tufts University.

, program director of Tissue Engineering at NIBIB. “It combines a deep understand of brain physiology with a large and growing suite of bioengineering tools to create an environment that is both necessary and sufficient to mimic brain function.”

The key to generating the brain-like tissue was the creation of a novel composite structure that consisted of two biomaterials with different physical properties: a spongy scaffold made out of silk protein and a softer, collagen-based gel. The scaffold served as a structure onto which neurons could anchor themselves, and the gel encouraged axons to grow through it.

To achieve grey-white matter compartmentalization, the researchers cut the spongy scaffold into a donut shape and populated it with rat neurons. They then filled the middle of the donut with the collagen-based gel, which subsequently permeated the scaffold. In just a few days, the neurons formed functional networks around the pores of the scaffold, and sent longer axon projections through the center gel to connect with neurons on the opposite side of the donut. The result was a distinct white matter region (containing mostly cellular projections, the axons) formed in the center of the donut that was separate from the surrounding grey matter (where the cell bodies were concentrated).

Over a period of several weeks, the researchers conducted experiments to determine the health and function of the neurons growing in their 3D brain-like tissue and to compare them with neurons grown in a collagen gel-only environment or in a 2D dish.

The researchers found that the neurons in the 3D brain-like tissues had higher expression of genes involved in neuron growth and function. In addition, the neurons grown in the 3D brain-like tissue maintained stable metabolic activity for up to five weeks, while the health of neurons grown in the gel-only environment began to deteriorate within 24 hours. In regard to function, neurons in the 3D brain-like tissue exhibited electrical activity and responsiveness that mimic signals seen in the intact brain, including a typical electrophysiological response pattern to a neurotoxin.

Because the 3D brain-like tissue displays physical properties similar to rodent brain tissue, the researchers sought to determine whether they could use it to study traumatic brain injury. To simulate a traumatic brain injury, a weight was dropped onto the brain-like tissue from varying heights. The researchers then recorded changes in the neurons’ electrical and chemical activity, which proved similar to what is ordinarily observed in animal studies of traumatic brain injury.

Kaplan says the ability to study traumatic injury in a tissue model offers advantages over animal studies, in which measurements are delayed while the brain is being dissected and prepared for experiments.

“With the system we have, you can essentially track the tissue response to traumatic brain injury in real time,” said Kaplan. “Most importantly, you can also start to track repair and what happens over longer periods of time.”

Kaplan emphasized the importance of the brain-like tissue’s longevity for studying other brain disorders. “The fact that we can maintain this tissue for months in the lab means we can start to look at neurological diseases in ways that you can’t otherwise because you need long timeframes to study some of the key brain diseases,” he said.

Hunziker added, “Good models enable solid hypotheses that can be thoroughly tested. The hope is that use of this model could lead to an acceleration of therapies for brain dysfunction as well as offer a better way to study normal brain physiology.”

Kaplan and his team are looking into how they can make their tissue model more brain-like.  In this recent report, the researchers demonstrated that they can modify their donut scaffold so that it consists of six concentric rings, each able to be populated with different types of neurons. Such an arrangement would mimic the six layers of the human brain cortex, in which different types of neurons exist.

As part of the funding agreement for the Tissue Engineering Resource Center, NIBIB requires that new technologies generated at the center be shared with the greater biomedical research community.

“We look forward to building collaborations with other labs that want to build on this tissue model,” said Kaplan.

This work was supported by NIH’s National Institute of Biomedical Imaging and Bioengineering under award #EB002520

NIBIB’s mission is to improve health by leading the development and accelerating the application of biomedical technologies. The Institute is committed to integrating the physical and engineering sciences with the life sciences to advance basic research and medical care. NIBIB supports emerging technology research and development within its internal laboratories and through grants, collaborations, and training. More information is available at the NIBIB website:http://www.nibib.nih.gov.

Source :NIH